Effect of DEC1 gene expression on proliferation and invasion abilities of human esophageal cancer ECA109 cells
Qiu Juan
Zhang Shikun
Abstract：Objective To investigate the effect of DEC1 gene expression on proliferation and invasion abilities of human e-sophageal cancer ECA109 cells.Methods Human esophageal cancer ECA109 cells were selected and transfected with plasmid pc DNA3.1(-)/DEC1 (study group)and pcDNA3.1(-)(control group).The expression of DEC1 mRNA was evaluated by real -time PCR,and the protein of cyclin D1,MMP9 and DEC1 were evaluated by Western blot.The cell proliferation and inva-sion ability were evaluated by Transwell test,colony formation assay and CCK -8 assay.Results The expression levels of DEC1 mRNA and DEC1 in the study group were significantly higher than those in the control group(P <0.05),but the expression lev-els of cyclin D1 and MMP9 were significantly lower than that of the control group(P <0.05).The cell proliferation and invasion ability of the study group was lower than that of the control group(P <0.05).Conclusion The expression level of DEC1 may affect the proliferation and invasion of human esophageal cancer ECA109 cells,and the inhibition of cyclin D1 and MMP9 expres-sion may be the mechanism of its action.
Keywords：esophageal cancerDEC1 geneECA109 cellsproliferationinvasion
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 1161-1163 )