Screening differential expression genes of primary hepatocellular carcinoma patients by gene chip technology
Dong Yahui
Song Zhan
Abstract：Objective To study the related gene expression in hepatic carcinoma of different stages (cancerous tissue,para-neoplastic tissue,normal tissue)by cDNA expression profiling microarray technique,and to further seek differentially expressed genes of hepatocellular carcinoma tissue in different stages.Methods The whole RNA of 20 cases of cancerous tissue,paraneo-plastic tissue,normal tissue of hepatic carcinoma was extracted.Agilent human genome 4 ×44K gene chip was applied to screen differentially expressed genes,and microarray analysis software (SAS software)was used to analyze chip image.Results 4 1 75 kinds of differentially expressed genes of cancerous tissue and normal mucosa tissue were screened,among which,there were 1 850 kinds of up -regulated genes and 2 325 kinds of down -regulated genes.Then through GO classification,screened differ-entially expressed genes mainly were divided into the following function and process:catalytic activity,signal transduction,enzy-matic activity regulation,transcription activity regulation,protein transportation function,cell growth and apoptosis,and so on. Conclusion Gene chip technology can high -throughput screen differentially expressed genes of hepatocellular carcinoma in dif-ferent stages,and provide experimental basis for further expounding the occurrence mechanism of hepatocellular carcinoma.
Keywords：hepatic carcinomagene expression profilinggene chip
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 393-395 )