Expression and correlation of ATG5,Beclin1 and P53 in epithelial ovarian carcinoma

Cao Meng; Zhao Shujun; Guo Huanhuan; Liu Xingshuo; Han Huihui; Zhang Min; Li Hongyu

doi:10.3969/j.issn.1004-437X.2015.10.001

Abstract：Objective To detect the expression of autophagy related gene ATG5,Beclin1 and apoptosis -related gene P53 in epithelial ovarian cancer,benign ovarian tumor and normal ovarian,and investigate their correlation and significance in the oc-currence and development of ovarian cancer.Methods Immunohistochemistry SP and RT -PCR methods were used to detect the expression level of ATG5,Beclin1 and P53 protein and mRNA in 25 cases of normal ovarian,25 cases of benign ovarian tumor and 34 cases of epithelial ovarian cancer tissue.And it was analyzed in combination with clinical pathological factors.Re-sults ①ATG5,Beclin1 mainly expressed in the cytoplasm,P53 mainly expressed in the nucleus.The positive expression rates of ATG5,Beclin1 in normal ovarian tissue,benign ovarian tumor and ovarian carcinoma were statistically different (P <0.05). The positive expression rates of P53 in ovarian cancer was different from benign ovarian tissue,normal ovarian tissue (P <0.01),but the expression rates between benign ovarian tissue and normal ovarian tissue were not statistically different (P >0.05).The expression rates of ATG5,Beclin1 and P53 were different in ovarian cancer tissues with different clinical and patholog-ical staging (P <0.05).Spearman correlation analysis showed that the expression of protein Beclin1 and ATG5 in epithelial ovarian carcinoma had a positive correlation;ATG5 and P53,Beclin1 and P53 protein expression had a negative correlation (P <0.05).②By RT -PCR,the positive expression rates of ATG5,Beclin1 in epithelial ovarian cancer tissues were lower than benign ovarian tumors and normal ovarian tissue;the positive expression rates in benign ovarian tissue were lower than normal ovarian tissue,and the differences were statistically significant (P <0.05).The expression level of P53 in epithelial ovarian cancer tissues was higher than benign ovarian tissue and normal tissue (P <0.05),but there was no difference between benign ovarian tissue and normal o-varian tissue (P >0.05).Conclusion The occurrence and development of epithelial ovarian cancer may be associated with the in-hibition of the activity of autophagy -related genes Beclin1 and ATG5.Autophagy -related genes ATG5,Beclin1 and apoptosis -re-lated genes P53 may play an antagonistic role in the development of epithelial ovarian cancer. Combined detection is helpful to understand and judge the degree of progression of epithelial ovarian cancer,prognosis and guide clinical treatment.