Establishment of the experimental autoimmune encephalomyelitis(EAE) model in C57BL/6J mice
Huang Xiabing
Kang Qiaozhen
Wang Xiaodong
Ding Cong
Jiang Hui
Liu Shimeng
Liu Xin
Abstract：Objective To obtain chronic progressive EAE model used to study the pathogenesis and identify the novel drug target of multiple sclerosis. Methods The C57BL/ 6J mice were immunized with immunogen MOG35 - 55 by multi - point subcutane-ous injection in the hind flank. The clinical score assessment was performed daily according to Benson’s scoring criteria. And the histological examination of H&E - stained paraffin - embedded sections of spinal cord and brain harvested at peak of EAE was performed. Results A stable EAE model was successfully estab-lished with high incidence. Compared with the normal mice,inflammatory cell infiltrates were increased in the spinal cord and brain tissue of EAE mice. Conclusion We successfully established the MOG35 - 55 - induced EAE model in C57BL/ 6J mice.
Keywords：multiple sclerosisexperimental autoimmune encephalomyelitisMOG35 - 55
Publication Date：2015-08-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 8-10 )