rMBP - NAP suppress the tumor growth in H22 hepatic carcinoma - bearing mice through the IL -12/IL -23 axis
Wang Xiaolong
Kang Qiaozhen
Men Yingli
Huang Xiabing
Zhang Yameng
Liu Xin
Abstract：Objective To study the antitumor effect and action mechanism of rMBP - NAP in hepatic cancer H22 bearing mice. Methods 2 × 106 H22 hepatoma cells were injected subcutaneously into the left flank of BABL/ c mice. On the next day, mice were divided into two groups and treated with PBS and rMBP - NAP,respectively. The tumor growth was monitored every day. The splenocytes from tumor bearing mice were cocultured with MMC - treated H22 tumor cells in the presence of rMBP -NAP,and anti - IL - 12 or anti - IL - 23 antibodies were added immediately. The secretion of IFN - γ in the supernatant were detected with ELISA assay 3 and 7 days later. Results Compared to PBS - treated mice,rMBP - NAP treatment significant-ly inhibited tumor growth. Moreover,the secretion of IFN - γ of splenocytes from tumor bearing mice was significantly in-creased with stimulation of rMBP - NAP in vitro. And it decreased significantly after addition of anti - IL - 12 or anti - IL -23 antibodies 3 and 7 days later. Conclusion Immunoregulatory agent rMBP - NAP can significantly inhibit hepatic canc-er H22 growth in mice.
Keywords：rMBP - NAPIL - 12IL - 23immunoregulato-ry agentIFN - γ
Publication Date：2015-08-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )