The Influences of extending incubate time of different experimental steps on the results of HBsAg enzyme -linked immunosorbent assay
Yan Fenghao
Zhong Zhanhua
Zeng Shaoli
Chen Yuan
Abstract：Objective To investigate the influences of extending incubate time of different experimental steps on the results of HBsAg enzyme -linked immunosorbent assay.Methods Without changing the condition of other experimental parameters,the incubate time before and after dispending enzyme and coloration were extended.The absorbances in the strong positive,positive, weakly positive and negative samples were detected and analyzed with SPSS 1 7.0 software.Results ①Extending the incubate time before dispending the enzyme had a statistical difference between the strong positive and weakly positive samples.The absor-bances of strong positive samples were obviously decreased while weakly positive samples were improved remarkably,and it was closely related to the time.②Extending the incubate time after dispending the enzyme had the most significant influences on each specimen except the negative one.③Extending the coloration time did not have obvious influence on the experimental results.④Extending the incubate time of different experimental steps had no statistical influence on the absorbance of the HBsAg negative samples.Conclusion Extending the incubate time of the right steps could improve the accuracy and the detective rate of weakly positive sample in ELISA experiments.
Keywords：enzyme -linked immunosorbent assayincubate timeabsorbancedetective rate
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 16-18 )