Cytogenetic analysis of childhood acute leukemia
Chen Yin
Xu Xueju
Abstract：Objective To study the cytogenetic features of childhood acute leukemia.Methods 100 cases of acute leukemia were enrolled in the study from October of 2013 to October of 2014 in the First Affiliated Hospital of Zhengzhou University.The genetic informations of bone marrow or blood were analyzed.Results There were only 76 cases who had mitosis,4 cases without splitting,45 cases with cytogenetical abnormalities.The rate of chromosomal abnormality was 59.21%,numeral chromosome ab-normalities accounted for 48.89%(22 /76),while structural abnormalities accounted for 37.78%(17 /76),6 cases had the both abnormalities,cytogenetical abnormalities were found in 15 cases of AML.The rate of chromosomal abnormality was 75.00%. The detection rate of gene fusion was 42%(42 /100).TEL/AML1,PML/RARa,E2A /PBX1,AML1 /ETO,MLL fusion genes and BCR/ABL genes were observed in 15,7,6,4,3 and 2 cases respectively.The multiplex RT -PCR in combination with chromosome analysis could detect genetic abnormalities in 69%.Conclusion Childhood acute leukemia had unique cellular and molecular genetic characteristics.The combination of assay of multiplex RT -PCR and karyotypic analysis was useful for the di-agnosis of AL,the evaluation of therapeutic effects and prognosis.
Keywords：acute leukemiachromosome analysisRT -PCR
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 31-33 )