The establishment for orthotopic implantation model of human glioma cells in mude mice
BU Xing-yao
ZHANG Feng
ZHANG Xiang
Walter E. Laug
Abstract：Objective: To explore the establishment for orthotopic implantation model of human glioma cells in nude mice. Methods: The human brain glioma cell line U87 MG was cultured in vitro, cells resuspended in serum-free RPMI 1640 medium at 108/ml were inoculated by injection with stereotactic guidance into the frontal white matter of nude mice. General observation were conducted after injection.The animals were sacrified at intervals from one hour to 63 days after implantation and the brain examined by pathology and immunohistochemistry and tumor size measured. Results: Optimal injection rate was found to be 0.05 μl/min = 1 ul/20 min, a volume of 1 ul containing 105 cells and injection was done over 20 min. Tumor cells form a nice sphere in the caudate putamen, away from ventricle as well as brain surface with no noticeable reflux into the needle track. Gliomas were formed in 100% in nude mice, and the growth of tumors was stable. The tumor growth in the regions of intracerebral environment in which tumors showed the morphological features of human glioma. Conclusion: The method described allows the highly accurate and reproducible introduction of a given number of cells into a specific area of the nude mice brain. The glioma model in nude mice is reliable animal model for the study of the tumorigenesis and biological characteristics and therapy.
Keywords：Gliomaxenograftcell cultureanimal modelnude mice
Publication Date：2005-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 289-294 )