The study of oral epithelium culture in vitro
QIU Chang-hong
ZHAI Hong-feng
LI Sen-kai
Abstract：Objective：In this article we study the technique and method of oral epithelium culture in vitro,playing a basic role in further study in reconstructing a tissue engineering oral mucosa and providing an experimental model in physiological,pathological,toxicological and microbiological study in oral mucosa.Methods：Taking a bit of oral mucosa from a young male New Zeal and hare who has just been out of milk,we digest it into single cell suspension with enzyme.Being seeded,the cells are cultured motionlessly.The medium is changed regularly and the cells are subcultured. Results：The cultured cells are all epithelial cells without fi broblasts.What is more,they are proved to be diploid cells.The cells can be subc ultured 11-13 generations,surviving 50-60 days. Conclusion: The oral epithelium of young New Zealand hare can be cultured in vitro,maintaining the ability to proliferate in a certain time.The study not only sets a role in reconstructing a tissue engineering oral mucosa,but also provides an experimental model for the research of oral mucosa in vitro.
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Publication Date：2001-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 112-115 )