Study on diagnostic value of common positive of 5 kinds of methodscombination for detection of smear and culture negative pulmonary tuberculosis
Abstract：Objective: To evaluate the diagnostic value of common positive of 5 kinds of methods(sputum PCR TB-DNA, serumLAM IgG, PPD IgG, SCIC and PPD 0.1 U skin test) used jointly for smear and culture negative pulmonary tuberculosis. Meth-ods: smear and culture positive cases 31,healthy control cases 53,non-tuberculosis Iung disease cases 30,smear and culturenegative cases 54. Methods were used for every case synchronousely The sputum were detection with PCR-TB-DNA, serum withELISA and PPD with skin test. Results: The positive rate(2,3,4,5 methods common positive)is 0,specificity is 100% in thehealth control group; the common positive rates among 2,3,4,5 methods respectively are 67.2% ,58.1% ,32.3% ,0% insmear and culture positive group; 45.9%, 18.9%, 2.7%, 0% in smear and culture negative group and 21.7%, 13.0%,0% ,0% in the non-tuberculosis pulmonary diseases group. Conclusion: The specificity of common positive rate of combina-tion detection of 5 kinds of methods is 100% in healthy control group; in smear and culture negative group the positive rate ofany 2 combination detections among 5 methods is 45.9% ,higher than any single detection of 5 methods. So those methods areavailable to smear and culture negative tuberculous diagnosis.
Keywords：tuberculosispulmonarydiagnosispolymerase chain reactionenzyme-linked immunosorbent assaytuberlin test
Publication Date：2000-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 315-317 )