Study on the mutagenicity of Penicillium digitatum
Abstract：Objective: We studied the mutagenicity of the extract from Penicillium digitatum. Methods: Revere mutation as-say, micronucleus test,unscheduled DNA syntnesis (UDS) in the primary cells of the lung and liver of rat and E. coli K12 in-fA gene mutation assay(the sequences of mutated infA gene were detemined and then analyzed by computer). Results: ①Theextract induced the reverse mutation of E. coli ND-160 strain;②The extract significantly did the increase of micronucleus rateof mouse bone marrow PCE(P＜0.01);③The extract could induce UDS in the primary cells of the lung and liver of rat,andUDS was particularly obvious in the primary cells of lung ( P＜0.01 ); ④Five points mutation in nucleotide of infA gene wereinduced by the extract and one of them led to amino acid variation(Lys→Val). Conclusion: Penicillium digitum could causegene mutation.
Keywords：Penicillium digitatumreverse mutationmicronucleusunscheduled DNA synthesisE coli K12 infA gene
Publication Date：2000-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 304-307 )