Clinical and experimental study on hypertension related to r-HuEPO therapy
Abstract：Objective: To investigate the pathogenesis of r-HuEPO-related hypertension. Methods: Changes of nitric oxide(NO) production and endothelin-l(ET-1) were observed in anemic patients with chronic renal failure (CRF) during hemodial-vsis (HD),anemic rats with CRF after 16 weeks of r-HuEPO treatment resulting in hypertension and control groups. We alsodid the similar detection in patients with hypertension during HD. The levels of plasma NO metabolites (NO2-/NO3- ) and ET-1 were determined. Then inducible NO synthase mRNA (iNOS mRNA) was detected by RT-PCR in rats' renal cortical andmedulla. Results: Hct,BP and the levels of plasma ET-1 were significantly higher(P＜0.01),but the levels of plasma NOwere markedly lower ( P＜0.05 ). There were no changes of these in control groups ( P＞0.05). Othermore, the level of plasmaNO2-/NO3- was increased and ET-1 was decreased after HD iNOS mRNA level was obviously lower in CRF rats than that ofcontrol rats. Conclusion: The decreases of iNOS mRNA and the increases of ET-1 play an important role in the development ofhypertension relating to r-HuEPO therapy.
Keywords：r-HuEPOCRFanemiahypertensionnitric oxideendothelin-1
Publication Date：2000-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 293-295 )