Study on value of cytophotometric determination of and DNA content of tumor cells in diagnosis and prognostic evalootion
Abstract：Objective: To study the value of cytophotometric determination of cell nuclear DNA in diagnosis and prognosis oftumor. Methods: The neuclear DNA content of tumor cells in 15 patients with salivary tumor, 17 patients with malignant lym-phoma,23 patients with early gastric cancer and 20 patients with early superficial esophageal cancer were measured by using animproved DNA-Feulgen staining and under OPTON type Ⅲ microspectrophotometer. The results of measurement of DNA con-tent were compared with the histopathological diagnosis and the follow-up data. Results: The increased neuclear DNA content,widespread DNA distribution and aneuploid DNA pattern were found in malignant tumor cells. The recurrence rate of tumor was significantly higher in esophageal cancer with aneuploid DNA pattern than in diploid tumor (P<0.05). Condusion: Cy-tophotometric determination of DNA content could provide an objective basis for differential diagnosis of benign or malignant tu-mor. DNA ploidy may be a valuable parameter for prognostic evaluation of esophageal cancer. Moreover, the effect of DNA-Feul-gen staining improved by us is very good.
Keywords：rumorDNAcytophotometrydiagnosis
Publication Date：2000-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 130-131,136 )