Molecular typing of the major outer membrane protein gene ofCampylobacterjejuni isolates from patients with Guillain-Barré syndrome
MA Jian-Jun
XU Jun
Nishimura Masataka
Saida Takahiko
Abstract：Objective: To study thd momecular typing of the major outer membrane protein gene(momp) of Campylobacter jejuni(C.jejuni). Methods: The C. jejuni major outer membrane protein gene was arnplified by PCR from a total of 163 strains. AfterHind Ⅲ , HaeⅢ, Mbo Ⅱ and HhaI restriction enzyme digestions, the digest was analyzed by electrophoresis, and then comparedwith heat-stable(HS) serotype and PCR-based restriction fragnent length polymorphism(RFLP) typing of flagellin A gene (flaA). Results: Eight distinct momp RFLP genotypes were observed. Among all of them, one major type (momp-3) accounted for61% of all isolates. All 34 HS-19:Cj(fla)-1 strains,including isolates from patients with Guillain-Barré syndrome(GBS) and en-teritis,belonged to a single genotype(momp-1). Conclusion: The HS-19:Cj(fla)-1 :momp-1 strain is distinctive among C.jejunistains,and HS-19 related GBS may be an excellent model to elucidate the pathogenesis of GBS.
Keywords：Guillain-Barré syndromeCampylobacter jejuniPCRmajor outer membrane proteingene
Publication Date：2000-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 100-102,106 )