Effects of Combined Application of Slow-Release Fertilizer and Bio-organic Fertilizer on the Growth,Yield and Quality of Stevia rebaudiana

Lei Yongqi; Tang Heyi; Wang Ge; Wu Yingxia; Xu Xinjuan

doi:10.15933/j.cnki.1004-3268.2026.08.008

Abstract：To clarify the synergistic improvement mechanism of slow-release fertilizer combined with bio-organic fertilizer and explore the green and efficient fertilization mode of Stevia rebaudiana,single slow-release fertilizer was used as the control(CK),and ordinary organic fertilizer(OF)and bio-organic fertilizer(BOF)were separately applied on the basis of equal amount of slow-release fertilizer.The effects of combined application of slow-release fertilizer and fertilizer on soil physicochemical properties,individual plant nutrient accumulation,photosynthetic physiology,stress resistance characteristics,yield,and steviol glycoside content of Stevia rebaudiana were systematically investigated.The results showed that the application of ordinary organic fertilizer or bio-organic fertilizer had no significant effect on soil pH value,but significantly reduced soil electrical conductivity(EC)value.Among them,the application of bio-organic fertilizer had the lowest EC value,which was 9.19%lower than the control.The contents of soil organic matter,alkali-hydrolyzable nitrogen,and available phosphorus were the highest when combined with bio-organic fertilizer,increasing by 21.59%,5.38%,and 21.57%respectively compared to the control.The single plant with the application of bio-organic fertilizer had the highest nutrient accumulation,with leaf nitrogen,phosphorus,and potassium accumulation increasing by 73.21%,84.24%,and 110.87%respectively compared to the control.The application of bio-organic fertilizer significantly increased leaf chlorophyll a+b content,net photosynthetic rate,protein content,and proline content by 51.02%,8.69%,32.76%,and 47.33%,respectively compared to the control.It significantly reduced the content of malondialdehyde in the leaves,which was 12.30%lower than the control.Combined application of bio-organic fertilizer significantly increased the dry weights of roots,stems,leaves and yield of Stevia rebaudiana,with a 77.48%increase in yield compared to the control.Except dulcoside(DA),the accumulation of leaf glycosides was also highest in the treatment with combined application of bio-organic fertilizer.The main glycoside components,stevioside(STV)and rebaudioside A(RA),increased by 59.41%and 59.55%respectively compared to the control.The correlation analysis results showed that yield was significantly negatively correlated with soil EC value and total potassium content of roots(TKR),and significantly positively correlated with soil alkaline-nitrogen(AN)content,leaf phosphorus(TPL)content,potassium(TKL)content,chlorophyll(a,b,a+b)content,net photosynthetic rate(Pn),protein(Pr)content,and proline(Pro)content;all glycoside components were significantly negatively correlated with total potassium content in leaves(TKL).In summary,the combination of slow-release fertilizer and bio-organic fertilizer can improve soil nutrient status,promote plant photosynthesis and stress resistance,thereby increasing the yield and glycosidic accumulation of Stevia rebaudiana.