Study on Essential Oil Extraction from Different Parts of Ocimum×citriodorum and Its Cigarette Flavoring Effect

CHEN Haoran; WANG Liusheng; LI Xinyan; FENG Wenning; LIU Wei; LI Yuanyuan; ZHAO Mingqin

doi:10.15933/j.cnki.1004-3268.2026.06.014

Abstract：To explore the differences in essential oil components in different parts(stem,leaf and whole plant)of Ocimum×citriodorum and develop natural characteristic tobacco flavorings,three extraction methods,including ultrasonic extraction,soxhlet extraction and simultaneous distillation and extraction,were adopted to extract Ocimum×citriodorum essential oil.The chemical compositions and aroma characteristics of essential oils from different parts were systematically analyzed,and their flavoring effects in cigarettes were evaluated.The results showed that 20,15 and 40 volatile components were extracted by ultrasonic extraction,soxhlet extraction and simultaneous distillation and extraction,respectively.The extracts obtained by the first two methods were mainly composed of acids,with relative contents of 45.35% and 44.19%,respectively.However,the extract of simultaneous distillation and extraction was dominated by olefins,with a relative content of 24.25%,and the most volatile components were detected.Essential oils were extracted from fresh and dried Ocimum×citriodorum by simultaneous distillation and extraction.The results indicated that the extraction rate of fresh samples was higher than that of dried samples,and the extraction rate of leaves was higher than that of stems and whole plants.A total of 31,29 and 36 major volatile components were extracted in essential oils from fresh stems,leaves and whole plants of Ocimum×citriodorum,while 29,29 and 40 major components were detected in dried samples,respectively.Olefins and alcohols were abundant in all samples in terms of component species.A total of 10 key aroma substances with relative odor activity value≥1 were screened from essential oils of different Ocimum×citriodorum parts,including 1-octen-3-ol,linalool,geraniol,eugenol,(Z)-citral,citral,(E)-citral,phenylacetaldehyde,β-ionone and β-caryophyllene.All samples presented distinct herbal and fresh aroma notes.The cigarette flavoring and smoking evaluation results showed that the fresh leaf essential oil achieved the highest sensory score of 29.0 at the addition level of 0.2 mg/cig,which could endow cigarettes with a fresh sweet aroma.In conclusion,the fresh leaf essential oil of Ocimum×citriodorum at the addition dosage of 0.2 mg/cig exhibited the optimal flavoring effect on cigarettes.It could improve aroma quality and aroma intensity,as well as enhance smoking taste.