Identification of VvABF Gene Family and Expression Analysis under Post-harvest ClO2 Treatment in Shine-Muscat Grape
YUAN Yilin
YUE Hong
WANG Liangrui
LI Yuxuan
ZHENG Pufan
CHEN Ting
MIAO Yungang
LI Xiaoxue
TAN Liping
Abstract：Abscisic acid(ABA)-responsive element(ABRE)binding factor proteins(ABF)constitute a crucial subfamily of bZIP transcription factors that play pivotal roles in both ABA-dependent and ABA-independent signaling pathways.Through bioinformatics approaches,members of the VvABF family were identified from transcriptome sequencing data of Shine-Muscat grape,followed by bioinformatics analysis.Their expression patterns under different storage days and varying chlorine dioxide(ClO2)concentration treatments were further investigated using qRT-PCR analysis.The results showed that the VvABF family comprised thirteen members dividing into three subfamilies and each member contained three to six exons,and the VvABF family members distributed across ten chromosomes with four gene pairs exhibiting collinear relationships.The VvABF family proteins were all hydrophilic and unstable.The entire VvABF family had seven conserved motifs and six conserved structural domains superfamily,which might interact with non-specific serine/threonine protein kinases such as D7TPP4_VITVI.Notably,8 g/kg ClO2 treatment significantly increased the expression levels of VvABF2,VvABF4,VvABF9,and VvABF13.ClO2 may mitigate post-harvest browning and aging of Shine-Muscat grape fruits by increasing the expression levels of VvABF2,VvABF4,VvABF9,and VvABF13 genes.
Keywords：Shine-Muscat grapeABF transcription factorPost-harvest storage qualityClO2 preservation treatmentTranscriptome sequencingBioinformatics analysis
Publication Date：2026-06-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：12( 120-131 )