Effects of Different Vernalization Methods and Durations on the Vernalization Effect of Late-Bolting Common Chinese Cabbage

DAI Ruifang; SHENG Lu; TAN Shuting; SONG Shixia; LIU Dan; ZHOU Xiaobo; AI Xin

doi:10.15933/j.cnki.1004-3268.2026.06.010

Abstract：To investigate the effects of different vernalization methods and durations on the vernalization of late-bolting common Chinese cabbage,the inbred lines E-67 and E-50,which exhibit varying levels of bolting resistance,and the control variety Shanghai May Slow were used as experimental materials.Under low-temperature conditions of 4℃,three vernalization methods including green tissue vernalization,light-exposed seedling vernalization,and seedling vernalization were applied,along with four vernalization duration gradients of 60,50,40,30 days.We systematically analyzed the effects of these treatments on key indicators of late-bolting Chinese cabbage,including bud emergence rate,bud emergence time,breeding effect,and plant growth status.The results indicated that under different vernalization methods,the bud emergence time tended to occur earlier as the duration of low-temperature vernalization increased,with significant differences observed between varieties(lines).The effects of green tissue vernalization and light-exposed seedling vernalization were similar,and both significantly shortened the time required for vernalization compared to traditional seedling vernalization.Plants subjected to green-tissue vernalization and light-exposed seedling vernalization exhibited robust growth and higher-quality bolting;in contrast,seedling vernalization,compared to the other two methods,more readily highlighted differences in bud emergence time among individual plants within a line,facilitating the selection of superior late-bolting individuals.In summary,green tissue vernalization and light-exposed seedling vernalization are beneficial for accelerating the breeding process of late-bolting common Chinese cabbage,while seedling vernalization offers distinct advantages in the selection of superior late-bolting individual plants.