Characterization and Expression Analysis of SWI3 Gene Family from Grape

CUI Xiaoyue; LÜ Zhongwei; SHANG Hongquan; MA Kaixin; LOU Yusui; ZHANG Ke; LU Huiran; WU Wenying; FAN Hongjie

doi:10.15933/j.cnki.1004-3268.2026.04.011

Abstract：The chromatin remodeling complex SWI/SNF plays an important role in plant growth and development and response to stresses.The characteristics of grape SWI3 gene family were systematically studied by bioinformatics analysis.In this study,four SWI3 gene family members,VvSWI3A,VvSWI3B,VvSWI3C,and VvSWI3D,were identified from Vitis vinifera,which were distributed on three chromosomes of grape.The results of physicochemical properties analysis showed that the four VvSWI3 proteins were acidic hydrophilic proteins with nuclear localization,and α-helix and random coil accounted for a large proportion(86.59％—94.27％)in the secondary structure.VvSWI3 proteins were divided into four subfamilies based on phylogenetic analysis,each of which contained one member.All VvSWI3 proteins contained SWIRM,SANT,and SWIRM_assoc_1 domains.In addition,VvSWI3D also contained a Zinc finger-ZZ type domain.The four VvSWI3 genes were similar in structure and contained 5-8 introns.The results of collinearity analysis showed that European grapes had experienced five gene replication events related to the VvSWI3 family during evolution.Different numbers of light,hormone,stress,growth,and development response elements existed in the promoters of the VvSWI3 gene family.It was speculated that the VvSWI3 genes might be involved in the regulation of grape growth and development,stress,and hormone response.It was found that VvSWI3 genes mainly expressed in grape leaves,could respond to drought,low temperature,and high temperature stresses,and showed different expression patterns after ABA,SA,MeJA,and ethephon treatment.