Identification of GATA Family Members in Momordica charantia and Analysis of Their Functions under Salt Stress
ZHANG Xiaoxia
WANG Jinku
FENG Songhao
WANG Yaxu
LOU Lifang
Abstract：Momordica charantia is a crop with significant economic and medicinal value.However,research on the distribution and stress resistance characteristics of the GATA gene family in Momordica charantia is still limited.This study identified and functionally analyzed the GATA gene family(McaGATA)in Momordica charantia through bioinformatics analysis.Results indicated that a total of 22 McaGATA genes were identified,distributed across 14 chromosomes,and divided into 4 subfamilies based on phylogenetic analysis.The analysis of the physicochemical properties of McaGATA proteins showed that there were differences in the number of amino acids,molecular mass,isoelectric point,etc.,but they were all hydrophilic nuclear proteins.Cis-acting element analysis revealed that the promoter region of the McaGATA genes were rich in light response,hormone response,and stress response elements.qRT-PCR analysis indicated that McaGATA genes presented tissue-specific expression patterns in different tissues,and genes such as McaGATA9,McaGATA10,McaGATA15,McaGATA17 and McaGATA18 were induced by salt stress,showing significantly upregulated expression under low-concentration salt stress conditions.Furthermore,the yeast model was used to verify that McaGATA4,McaGATA15,McaGATA17 and McaGATA18 genes could significantly enhance the salt tolerance of yeast cells under low-concentration salt stress,while McaGATA18 showed the most prominent salt tolerance effect under high salt stress.
Keywords：Momordica charantiaGATA gene familySalt stressYeast
Publication Date：2026-03-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：10( 61-70 )