Research on Biological Characteristics of Glaesserella parasuis Serotype 5 cpxAR Two-Component Gene Deletion Strain
XU Yindi
WANG Zhifang
CAI Xuwang
XU Xiaojuan
ZHANG Lixian
JIAO Wenqiang
ZHU Wenhao
LI Haili
YOU Yi
ZHANG Jiaqing
LEI Ya'nan
Abstract：To investigate the pathogenic mechanism of the cpxAR two-component gene in Glaesserella parasuis and screen for the deletion-attenuated strain of Glaesserella parasuis serotype 5,the cpxAR double gene deletion strain(ΔcpxAR)of Glaesserella parasuis serotype 5 clinical isolate(GPS5)was constructed,and the growth characteristics,biofilm formation ability,environmental stress resistance and pathogenicity to guinea pigs were systematically analyzed.The results showed that after cpxAR gene deletion,there was no significant difference in colony morphology and growth rate between ΔcpxAR and its parent strain GPS5,but the biofilm formation ability was significantly weakened,the resistance to osmotic pressure,heat shock,oxidative stress and alkalinity was significantly reduced,and the virulence to guinea pigs was also significantly reduced.In conclusion,the cpxAR gene has no obvious regulatory effect on the growth of GPS5,but it has an important regulatory effect on its biofilm formation,environmental stress resistance and virulence.It lays a foundation for further exploration of the biological functions of the cpxAR two-component system in Glaesserella parasuis and screening of attenuated strains of Glaesserella parasuis serotype 5.
Keywords：Glaesserella parasuis serotype 5cpxAR gene deletion strainGrowthBiofilmResistanceVirulence
Publication Date：2026-02-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：10( 126-135 )