Genome-wide Identification and Expression Analysis of TCP Transcription Factor Family in Melon
LI Hailun
GAO Ningning
GUO Yaomiao
SUN Dongling
WANG Yueling
XIE Kuixi
LI Pengfei
DUAN Shixiang
ZHAO Weixing
YANG Sen
Abstract：TCP gene family is a class of transcription factors unique to plants and closely related to different stages of plant growth and development.There are few reports on the TCP gene family in melon.In this study,the members of the TCP gene family in melon were identified through bioinformatics methods,and their physicochemical properties,subcellular localization,phylogenetic evolution,chromosomal location,gene structure,conserved motifs,cis-elements,miRNAs and gene expression patterns were analyzed.The results revealed that a total of 29 members were identified within the TCP gene family.All these members contained the TCP domain and were unevenly distributed across nine chromosomes.The amino acid numbers of melon TCP transcription factor family proteins ranged from 61 to 600 aa,the molecular weights ranged from 7 115.16 to 65 469.20 u,and the theoretical isoelectric points ranged from 5.62 to 10.06.Subcellular localization prediction analysis revealed that 28 TCP proteins were localized within the nucleus.According to the phylogenetic analysis,the family members could be divided into two subfamilies,Class Ⅰ and Class Ⅱ,and further subdivided into three subgroups:PCF,CYC/TB1 and CIN.The gene structures of CmTCPs were relatively simple,all members containing 1 or 2 exons,and only a few genes contained introns.All the 29 genes contained motif 1,and the Motifs contained by different subgroups were different.The cis-element prediction analysis showed that the promoter sequences of the melon TCP gene family were mainly composed of light response,hormone response,growth and development-related elements.The miRNA prediction analysis obtained 25 miRNAs interacting with 13 CmTCP genes.The analysis of RNA-Seq data of melon genome database showed that 26 CmTCP genes had both distinct tissue-specific expression and specific expression in certain tissue parts in melon tissues.The results of qRT-PCR indicated that the relative expression levels of CmTCP-CYC/TB1 gene family members in different tissues of melon were significantly different.
Keywords：MelonTCP gene familyBioinformatics analysisGenome-wide identificationGene expression analysis
Publication Date：2025-09-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：14( 127-140 )