Effect of the gacS Gene in Pseudomonas chlororaphis on the Biocontrol Efficacy against Wheat Take-all Disease
WANG Haodong
LU Yifang
YANG Shan
LIU Fengying
WANG Gang
Abstract：To investigate the role of the GacS-encoding gene,a component of the GacS/GacA two-component system,in the biocontrol of wheat take-all disease by Pseudomonas chlororaphis zm-1,a combination of bioinformatics,gene knockout,and gene complementation techniques was used to study the function of the gene gacS.The results showed that the zm-1 genome contained a gacS gene that was highly homologous to that found in the P.aeruginosa genome.The gacS gene knockout mutant strain ΔgacS exhibited 26.88 percent points reduction in antifungal activity and 52.44 percentage points decrease in biocontrol efficacy against wheat take-all compared to the wild-type strain zm-1,while the complementary strain restored both antifungal activity and biocontrol efficacy.The addition of 1-hydroxyphenazine,a secondary metabolite of zm-1 strain,restored the biocontrol efficacy of the ΔgacS mutant strain.However,the addition of 1-hydroxyphenazine to the wild-type strain zm-1 did not result in a synergistic effect.Taken together,these findings indicate that the gacS gene exists in the zm-1 genome and exerts biocontrol effects against wheat take-all disease by promoting the production of antimicrobial secondary metabolites.
Keywords：Wheat take-allBiological controlPseudomonas chlororaphisAntifungal substancesgacS gene
Publication Date：2025-09-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 112-118 )