Molecular Identification and Complete Sequence Analysis of Chilli Veinal Mottle Virus GZ-tobacco Isolate
ZHANG Changcheng
JIANG Jun
PAN Shouhui
XUE Yuan
ZHANG Quan
LI Zikuo
WANG Hancheng
SHI Caihua
FANG Shouguo
ZHANG Songbai
LI Xiquan
Abstract：In order to clarify the pathogen and its molecular characteristics,transmission mode and pathogenicity of tobacco new virus disease in Guizhou,and effectively prevent and control the virus disease,the pathogen was detected and identified by virus detection strip,electron microscope observation,indirect ELISA,RT-PCR and dsRNA technology.The whole genome sequence of the pathogen was amplified by RT-PCR,and the evolutionary relationship of the pathogen and the possible recombination events were analyzed by phylogenetic tree and recombination analysis.Through biological inoculation of the pathogen to N.benthamiana and Yunyan 87,the incidence and symptoms were observed.The results showed that the linear virus particles of(12-14)nm×(700-900)nm were observed in the juice of the diseased plants by electron microscopy.Potato virus Y(PVY)detection test strip was positive,but PVY specific primer RT-PCR was negative.The random clone fragments of the pathogen genome were obtained by dsRNA technology and sequenced as the Chilli veinal mottle virus(ChiVMV)genome fragments.The virus strains could be transmitted by root irrigation,friction and mediator.The typical symptoms of N.benthamiana were chlorosis,yellowing,blister spot,leaf margin curling and plant dwarfing.The typical symptoms of Yunyan 87 were mottle,blister spot and leaf margin curling.The genome sequence length of the strain was 9 782 nt(accession number:OP378160),which was the closest to ChiVMV YN-tobacco in phylogenetic relationship.Further recombination analysis showed that the strain might be a recombinant isolate.In summary,the new pathogen of tobacco disease in Guizhou is a new strain of ChiVMV,named ChiVMV Guizhou tobacco strain(GZ-tobacco),which can cause typical symptoms of tobacco virus disease.
Keywords：TobaccoChilli veinal mottle virusIsolateMolecular identificationBiological characteristicsSequence analysis
Publication Date：2025-08-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：9( 102-110 )