Study on the Differences in Growth and Metabolomics of Flavonoids in Tissue Culture Seedlings of Lilium pumilum DC.under Different Light Quality
DENG Yu
HOU Jia
MA Zhengzheng
LI Zhengxiao
LU Songhui
Abstract：Under the different light qualities,the differences in growth and flavonoid metabolites of L.pumilum DC.tissue cultured seedlings were analyzed.During the proliferation and growth stage of L.pumilum DC.tissue cultured seedlings,five light quality treatments were set up:white light(W),pure red light(R),pure blue light(B),red blue 1∶1 composite light(R1B1),and red blue green 1∶1∶1 composite light(RGB).We observed and compared the growth differences of each treatment,and detected flavonoids using UHPLC-MS/MS technology.Under W treatment,the proliferation coefficient of L.pumilum DC.was the highest,at 3.31,the overall growth was better,the leaves were more robust,and the cluster seedlings were more vigorous.Compared with white light quality,red light quality contained 5 differential flavonoids(R_W),2 differential flavonoids were screened out in the B_W,29 differential flavonoids were screened out in the R1B1_W,and 22 differential flavonoids were screened out in the RGB_W.The relative content of differential flavonoids was the highest under RGB treatment,and flavonoids with a higher proportion such as kaempferol and rutin were significantly upregulated.KEGG pathway analysis showed that these substances were mainly enriched in flavonoid and flavonol biosynthesis pathways and flavonoid biosynthesis pathways.What was more important,R1B1 indirectly inhibited the accumulation of kaempferol and downstream substances by regulating the precursor substance naringenin chalcone,while RGB directly positively regulated the accumulation of naringin and downstream flavonoids such as kaempferol.
Keywords：Lilium pumilum DC.Light qualityGrowthMetabolomicsFlavonoids
Publication Date：2025-07-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：10( 116-125 )