Identification of WOX Family Members in Salvia miltiorrhiza and Functional Analysis of SmWOX8 Gene under Salt Stress
CHEN Chun
ZHONG Chunbin
LU Lei
Abstract：This study identified the WOX family genes of Salvia miltiorrhiza(SmWOX)through genomic data,and analyzed their protein physicochemical properties,phylogenetic relationships,structure,gene tissue expression patterns,and response to salt stress.The results showed that 9 SmWOX family proteins had different physicochemical properties such as amino acid sequence length,molecular weight,and isoelectric point,and were all located in the nucleus.The results of systematic evolutionary analysis showed that SmWOX family members were divided into three groups:ancient,intermediate,and WUS,and had direct homologous relationships with some Arabidopsis WOX proteins.Structure analysis revealed the differences among members of different Salvia miltiorrhiza WOX subfamilies,which had different expression patterns in different tissues,suggesting that they may exercise different functions in growth and development.The analysis of cis-acting elements showed that the SmWOX genes had regulatory effects on stress,hormone response,and growth and development.In addition,there were interactions between SmWOX8 protein and other proteins.Under salt stress,the expression level of SmWOX genes changed significantly,especially SmWOX8,which had the highest expression level under high salt stress,suggesting that it may play an important role in salt stress resistance of Salvia miltiorrhiza.The results of transgenic Arabidopsis experiments confirmed that SmWOX8 could improve salt tolerance of Salvia miltiorrhiza.
Keywords：Salvia miltiorrhizaWOX gene familyExpression patternSalt stress
Publication Date：2025-06-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：12( 43-54 )