Effects of Combined Application of Boron,Zinc,and Magnesium Fertilizers on Growth,Yield and Quality of Stevia rebaudiana
YANG Chunyin
MA Fengjie
XIE Zhongqing
ZHANG Yaping
Abstract：Boron,zinc and magnesium fertilizers were selected for compounding,and 16 fertilization treatments were set up to analyze the effects of different treatments on agronomic traits,physiological indexes,glycoside content and yield of Stevia rebaudiana,which would provide scientific basis for spraying medium and micro fertilizers on Stevia rebaudiana leaves in Hexi area.The results showed that T7 treatment(the application amount of boron,zinc and magnesium fertilizers were 7.5,7.5,22.5 kg/ha,respectively)had the highest plant height and the largest number of branches,which were 21.2%and 92.5%higher than those of T16 treatment(control,without boron,zinc and magnesium fertilizers).In terms of biomass,the stem fresh weight of T4 treatment(the application amount of boron,zinc and magnesium fertilizers were 22.5,7.5 and 7.5 kg/ha,respectively)was the largest,which was 116.4%higher than that of the control.The fresh weight and dry weight of leaves treated with T7 were the highest,which increased by 139.6%and 125.1%,respectively,compared with the control.In terms of physiological indexes,the total chlorophyll content and superoxide dismutase(SOD)activity of T8 treatment(the application rates of boron,zinc and magnesium fertilizers were 7.5,7.5 and 7.5 kg/ha,respectively)were the highest,which were 18.3%and 15.7%higher than those of the control.In terms of glycoside accumulation,the content of rebaudioside A(RA)in T5 treatment(7.5,22.5,22.5 kg/ha of boron,zinc and magnesium fertilizers,respectively)was the highest,which was 26.2%higher than that of the control.The content of stevioside(Stv)in T2 treatment(boron,zinc,magnesium fertilizer application rates were 22.5,22.5,7.5 kg/ha)was the highest,which was 223.2%higher than that of the control.The total glycoside content of T8 treatment was the highest,which increased by 29.6%compared with the control.T7 treatment achieved synergistic high yield of RA(83.13 g/kg)and Stv(34.20 g/kg).The yield of T7 treatment reached 11 788.5 kg/ha,which was 125.1%higher than that of the control.Correlation analysis showed that RA content was significantly positively correlated with total chlorophyll content and SOD activity.Stv content was significantly negatively correlated with total chlorophyll content and SOD activity.There was a significant negative correlation between the content of total glycosides and SOD activity.Soluble protein content was significantly or extremely significantly positively correlated with total chlorophyll content,SOD activity and peroxidase(POD)activity.There was a significant positive correlation between soluble sugar content and POD activity.Analysis of variance showed that zinc fertilizer and magnesium fertilizer had extremely significant effects on RA and Stv contents,boron fertilizer and zinc fertilizer had extremely significant effects on plant height,and magnesium fertilizer had extremely significant effects on total chlorophyll content.Through the comprehensive evaluation of the membership function method,it was concluded that the T7 treatment effect was the best,that was,when the application rates of boron,zinc and magnesium fertilizers were 7.5,7.5 and 22.5 kg/ha respectively,it was most conducive to promoting the growth and quality promotion of Stevia rebaudiana.
Keywords：Stevia rebaudianaCompound fertilizer of boronzinc and magnesiumOutputQualityStevioside
Publication Date：2025-06-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：13( 30-42 )