The Application Effect of Immobilized Mushroom Strains of Pleurotus eryngii in Cultivation Production

ZHOU Tingting; JIANG Ran; ZHU Haoran; MA Guizhen

doi:10.15933/j.cnki.1004-3268.2025.05.014

Abstract：The preliminary research indicated that immobilized strains could still maintain high biological activity after storage at 20℃and 4℃for 30 days.In order to verify the application effect of immobilized strains in production practice,immobilized strains of different storage time were inoculated into Pleurotus eryngii cultivation bags,with fresh liquid strains as the control.The growth rate of Pleurotus eryngii mycelium,the time of mycelium filling bag,the contamination rate of cultivation bag,the total yield per cultivation bag at different storage periods were measured,and the biological efficiency and commodity rate were calculated.Nutrients such as ash,protein and fat were analyzed.The results indicated that the fruiting body morphology,cap diameter,stem diameter,and stem length of the cultivation bags inoculated with immobilized strains stored for 30 days at 20℃and 4℃showed no significant differences compared to the control.With the increase of storage time,the filling time of mycelium slowly increased,and the growth rate of mycelium gradually slowed down.The contamination rate of the bag was less affected by storage time and temperature.The total yield per cultivation bag was higher than 561.00 g/bag,the biological efficiency was higher than 122.00%,and the commodity rate exceeded 82.00%,meeting the production requirements.In addition,there were no significant differences in ash,protein,fat and other nutrients in the fruit body of Pleurotus eryngii.Therefore,the immobilized strains stored for 30 days can be used in the cultivation and production of Pleurotus eryngii,providing a feasible technical solution to the storage and transportation challenges of Pleurotus eryngii strains.