Effect of Phosphatidylinositol-3 Kinase Inhibitors on the Proliferation of Vesicular Stomatitis Virus in Vitro
WANG Mengdi
FAN Wenjie
ZHANG Zhen
ZHAO Ruihong
LI Huawei
LIU Kun
ZHENG Ming
WANG Yakai
Abstract：The aims are to study the effect of phosphatidylinositol-3 kinase inhibitor(PI3K-IN-1)on the proliferation of vesicular stomatitis virus(VSV)in vitro on PK-15 cell model,and to reveal the antiviral effect of PI3K-IN-1 on VSV in vitro,which will lay a foundation for seeking and developing drugs to prevent and control VSV infection.The safe concentration of PI3K-IN-1 at the cellular level was detected by CCK-8 method;flow cytometry was used to detect the effect of PI3K-IN-1 on the percentage of each period in the cell cycle and apoptosis;and flow cytometry and fluorescence microscopy were used to analyze the difference of virus proliferation in PK-15 cells infected with VSV after PI3K-IN-1 treatment;the mRNA expression level of VSV N gene was analyzed by RT-qPCR;the expression level of GFP protein in VSV-GFP was analyzed by immunoblotting;the effect of PI3K-IN-1 on the titer of VSV progeny virus was detected by virus titer assay;and the inhibitory effect of PI3K-IN-1 on the fluorescence intensity of GFP protein in VSV-GFP was analyzed by confocal microscopy.The results showed that 0-10 μmol/L PI3K-IN-1 had no effect on PK-15 cell viability,percentage of each period in the cell cycle and apoptosis rate.But with the increase of PI3K-IN-1 concentration,the expression level of GFP protein in VSV-GFP decreased.When the concentration of PI3K-IN-1 was 10 μmol/L,the inhibitory effect on VSV proliferation was the best.The relative expression level of VSV N gene mRNA in PI3K-IN-1 treatment group was significantly or extremely significantly lower than that in DMSO(CK)group and showed a time-dependent trend.At 12 h and 24 h after VSV-GFP infection,the proportion of GFP positive fluorescent cells in PI3K-IN-1 treatment group was extremely significantly lower than that in CK group,and the progeny virus titer of PI3K-IN-1 treatment group was significantly lower than that of CK at 12 h and 24 h after infection,and was extremely significantly lower at 36 h after infection.In summary,PI3K-IN-1 can effectively inhibit the proliferation of VSV in PK-15 cells,which provides a new strategy and method for the research and development of anti-VSV drugs.
Keywords：Vesicular stomatitis virusPhosphatidylinositol-3 kinase inhibitorAntivirusProliferation in vitro
Publication Date：2025-04-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：8( 136-143 )