Identification of the WOX Gene Family in Paeonia lactiflora Based on Transcriptome Data and Functional Analysis of PlWOX5 Under Salt Stress
PENG Bo
MA Mengmei
ZHAO Ping
Abstract：In order to clarify the function,evolutionary relationship,and role in biological processes of the Paeonia lactiflora WOX gene family,members of Paeonia lactiflora WOX family(PlWOXs)were identified based on transcriptome data,followed by a comprehensive bioinformatics analysis of their protein physicochemical properties,structures,evolutionary relationships,and expression patterns.The results showed that five PlWOX genes were identified based on the transcriptome data of Paeonia lactiflora,and their encoded amino acids were mainly hydrophobic and unstable alkaline nucleoproteins.The results of the system evolution analysis showed that the PlWOXs gene was distributed in both WUS/modern lineages and ancient lineages,with no PlWOXs gene distribution in the intermediate lineages.The results of protein structure analysis indicated that there were differences in the distribution and quantity of Motif on different PlWOXs genes,and they had significant tissue specificity.PlWOX2 and PlWOX5 were mainly highly expressed in Paeonia lactiflora leaves.The qRT-PCR detection results showed that PlWOXs genes were responsive to salt stress,but PlWOX5 showed a more significant response to salt stress.Overexpression of PlWOX5 gene could enhance salt tolerance in Arabidopsis by enhancing antioxidant enzyme activity(superoxide dismutase,peroxidase,catalase)and increasing the content of osmoregulatory substances(soluble proteins)to promote reactive oxygen species(ROS)clearance.In conclusion,PlWOX5 gene can improve the salt tolerance of Paeonia lactiflora by increasing the activity of antioxidant enzymes and the content of osmotic regulators.
Keywords：WOX gene familyPaeonia lactifloraSalt stressAntioxidant enzyme activityOsmotic regulatory substance
Publication Date：2025-04-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：9( 57-65 )