Characterisation of the NtHPL Genes and Their Expression Pattern under TMV Stress in Tobacco
WANG Mingxin
LIU Lei
DENG Zhaolong
WANG Haoyang
LIU Pengfei
JIN Weihuan
LI Junying
GUO Hongxiang
Abstract：Hydroperoxide lyase(HPL)is a key enzyme in the catalytic fatty acid oxidation pathway and belongs to the cytochrome P450(CytP450)superfamily.HPL plays an essential role in plant responses to both biotic and abiotic stresses,contributing significantly to the regulation of stress tolerance.In this study,the NtHPL gene family in tobacco was identified using genomic data and comprehensively analyzed for their physicochemical properties,protein structures,conserved motifs,and response patterns to tobacco mosaic virus(TMV).The results indicated that nine NtHPL family members were identified,distributed across eight chromosomes,and classified into three subclasses:B,C,and D.The amino acid lengths of these members ranged from 437 to 527 residues,with theoretical isoelectric points(pI)varying between 5.84 and 9.15.Subcellular localization predictions suggested that these proteins were primarily localized in chloroplasts and cytoplasm.Expression analysis revealed that upon TMV infection,five NtHPL genes were upregulated,one was downregulated,and three exhibited no significant differential expression in tobacco leaves.Overexpression of NtHPL1 enhanced tobacco resistance to TMV,as demonstrated by experimental evidence.These findings collectively suggest that NtHPL may be involved in the regulatory mechanisms underlying tobacco defense against TMV.
Keywords：TobaccoHydroperoxide lyaseTMVCytochrome P450
Publication Date：2025-04-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：10( 47-56 )