Identification and Functional Characterization of Endophytic Microbes from Mulberry Tree(Morus alba L.)

LI Sha; YANG Jiahu; WANG Yanhui; HUANG Xinqiu; YANG Wen

doi:10.15933/j.cnki.1004-3268.2025.03.009

Abstract：This study used methods such as microbial culture,target gene sequence amplification,and homology identification to isolate and identify endophytic microbes in Morus alba L.,and explored the strain types,distribution characteristics,and functional characteristics of endophytic bacteria and fungi in the roots,stems,and leaves of Yunsang No.6 mulberry.The results showed that a total of 43 endophytic bacteria belonging to 9 genera and 22 endophytic fungi belonging to 14 genera were isolated from mulberry trees.Among them,21 bacteria and 11 fungi were isolated from roots,13 bacteria and 5 fungi were isolated from stems,and 9 bacteria and 6 fungi were isolated from leaves.There were significant differences in the distribution of endophytic microbes in the roots,stems,and leaves of mulberry trees,there were Falsibacillus pallidus,Bacillus velezensis and Pseudomonas koreensis shared in the roots and stems of mulberry trees,Pantoea agglomerans,Bacillus subtilis and Pseudomonas koreensis shared in the stems and leaves,and Pseudomonas koreensis shared in the roots,stems,and leaves;Endophytic fungi Colletotrichum siamense was shared in mulberry roots and leaves,but no common fungi were found to exist in the stems and leaves,stems and roots.Further screening of functional microbes showed that 8 strains of nitrogen fixing microbes(7 bacteria,1 fungus),4 strains of phosphorus solubilizing microbes(2 bacteria,2 fungi),and 8 strains of potassium solubilizing microbes(6 bacteria,2 fungi)were obtained from 65 endophytic microbes,respectively.In summary,endophytic bacteria and fungi in mulberry trees exhibit diversity and distribution differences in roots,stems,and leaves.Among endophytic bacteria and fungi in mulberry trees,there are functional strains that can dissolve phosphorus,nitrogen,and potassium.