Effects of 6-BA on Physiological Characteristics of Primary Rhizomes of Polygonatum sibiricum Red.

WANG Rongrong; YUE Hewei; ZHANG Bo; HU Peng; LIU Yang; YANG Xue; LI Juan; LI Lianzhen

doi:10.15933/j.cnki.1004-3268.2025.03.007

Abstract：To investigate the effects of 6-benzylaminopurine(6-BA)on the physiological characteristics of the initial root tubers of Polygonatum sibiricum Red.,four mass concentrations of 6-BA(0 mg/L,CK;50 mg/L,M1;100 mg/L,M2;200 mg/L,M3)were applied to the initial root tubers.The germination and growth of the initial root tubers were observed,and the activities of peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),lipase,malate dehydrogenase(MDH),6-phosphogluconate dehydrogenase(G6PDH),and the contents of malondialdehyde(MDA),lipids,free amino acids,proteins,starch,and soluble sugars were measured.The results showed that the germination rate,seedling leaf length,leaf width,diameter,main root length,lateral root number,and dry weight of the primary rhizomes treated with 6-BA were significantly higher than those in the CK group(P＜0.05).On the 61st day,the emergence rates of M1,M2,M3,and CK were 33.10％,60.12％,46.66％and 3.68％;Among them,the M2 treatment showed significant differences compared to other treatments(P＜0.05).On days 1-31,the soluble sugar,starch,protein,free amino acids,and fat content in the primary rhizomes of Polygonatum sibiricum Red.treated with 6-BA were significantly lower than those in the CK group(P＜0.05),and the lipase activity was significantly higher than that in the CK group(P＜0.05);The activities of POD,SOD,CAT,MDH,and G6PDH enzymes in the primary rhizomes of Polygonatum sibiricum Red.treated with 6-BA were higher than those in the CK group,while the MDA content was lower.The activities of SOD,CAT,and G6PDH reached their highest values on the 41st day after treatment.Among them,the SOD activity in M1,M2,and M3 increased by 39.87％,86.99％,and 51.02％,respectively,compared to the CK group;The CAT activity in M1,M2,and M3 increased by 30.25％,54.06％,and 45.79％respectively compared to CK;The G6PDH activity in M1,M2,and M3 increased by 51.90％,132.42％,and 76.89％respectively compared to CK.The results of correlation and principal component analysis showed that the eight indicators of starch content,soluble sugar content,protein content,free amino acid content,fat content,peroxidase activity,superoxide dismutase activity,and catalase activity could be used as core evaluation indexes to characterize the emergence and growth of primary rhizomes of Polygonatum sibiricum Red.under different concentrations of 6-BA(0,50,100,200 mg/L).In conclusion,6-BA can significantly improve the emergence rate,substance metabolism level,and antioxidant enzyme activity of the primary rhizomes of Polygonatum sibiricum Red.,and the optimal concentration is 100 mg/L.