Effect of Spraying Amino Acid Chelated Zinc after Anthesis on Synthesis and Accumulation of Starch and Protein in Wheat Grains

ZHANG Bowen; JIN Haiyang; XU Haixia; ZHENG Fei; HE Ning; SU Yazhong; ZHAO Guojian; CHENG Hongjian; CHENG Xiyong; LI Xiangdong

doi:10.15933/j.cnki.1004-3268.2025.03.002

Abstract：Zinc sulfate,zinc aspartate acid and zinc glycinate of 0.2％and 0.4％were sprayed on wheat after anthesis with water as the control(CK),and the effects of spraying amino acid chelated zinc on the contents of soluble sugar,starch,amino acid and protein,and the activities of enzymes related to starch synthesis,nitrogen metabolism in wheat grains were studied in different periods,so as to identify the most efficient zinc fertilizer,and provide theoretical basis and technical reference for the high-yield and quality enhancement of wheat production.The results showed that compared with CK,spraying zinc glycinate and zinc aspartate significantly increased the activities of soluble starch synthase in grains at 10 days after spraying at Longting and Dancheng experimental sites,with increase rates of 16.26％—27.45％and 24.51％—39.13％,respectively;At Dancheng experimental site,spraying zinc sulfate,zinc glycinate and zinc aspartate significantly increased the activities of bound starch synthase in grains at 10 days after spraying,with increase rates of 9.93％—17.65％,while at Longting experimental site,only spraying 0.2％zinc aspartate significantly increased the activity of bound starch synthase in grains by 12.15％;Spraying zinc glycinate and zinc aspartate significantly increased the starch content in grains at 10 days after spraying at Longting and Dancheng experimental sites,with increase rates of 14.49％—20.82％and 13.26％—20.68％,respectively,there was no significant difference between zinc sulfate treatments and CK,and there was no significant difference in the starch content in grains at 20 days after spraying and mature stage between zinc treatments and CK.Compared with CK,spraying zinc glycinate significantly increased the activity of glutamine synthetase in grains at 10 days after spraying at Longting and Dancheng experimental sites,with increase rates of 16.67％and 10.42％—14.58％,respectively;Significantly increased the activity of glutamate synthetase in grains at 10 and 20 days after spraying at Dancheng experimental site,with increase rates of 12.18％—15.53％and 11.84％—12.76％,respectively.Spraying 0.2％zinc aspartate significantly increased the activities of glutamine synthetase and glutamate synthase in grains at 20 days after spraying at Longting experimental site,with increase rates of 23.21％and 10.83％,respectively.Spraying 0.2％zinc aspartate or 0.2％zinc glycinate significantly increased the protein content in grains at mature stage at both experimental sites,spraying 0.4％zinc glycinate only significantly increased the protein content in grains at mature stage at Dancheng experimental site,and 0.2％zinc aspartate treatment had the highest increase rates at both experimental sites,which were 7.90％and 7.25％,respectively.In conclusion,spraying 0.2％zinc aspartate,zinc glycinate after anthesis can significantly increase the protein synthesis and accumulation in wheat grains,improve the protein content in grains at mature stage,and 0.2％zinc aspartate has the best effect.