Correlation Analysis between Tissue Expression of CIDEa and CIDEc Genes and Intramuscular Fat Content in Huanghuai Sheep
LI Jun
GAO Fuxian
KUAI Ziru
QI Yuxin
HAN Haoyuan
SHI Huibin
LIU Kun
QUAN Kai
Abstract：The aim of this study was to explore the expression patterns of CIDEa and CIDEc genes in various tissues of Huanghuai sheep,and analyze their correlation with intramuscular fat and fatty acid content.This study selected three male Huanghuai sheep at the ages of 3,9,and 18 months each,collected tissue samples after slaughter and measured their intramuscular fat and fatty acid content.It used RT-qPCR technology to detect CIDEa and CIDEc genes expression level in various tissues and in longissimus dorsi at different ages,and analyzed their correlation with intramuscular fat and fatty acid content using Pearson correlation coefficient.The results showed that both CIDEa and CIDEc had highest expression levels in subcutaneous fat and abdominal fat.The expression level of CIDEa in 4 muscle tissues semitendinosus,longissimus dorsi,triceps brachii and quadriceps femoris increased overall with age.The expression level of CIDEa in the longissimus dorsi was positively correlated with intramuscular fat and C16:0(P<0.05),and negatively correlated with C18:0,C20:2,and C20:4n6(P<0.05).While the expression level of CIDEc gene in longissimus dorsi and triceps brachii decreased with age.The expression level of CIDEc in the longissimus dorsi was positively correlated with C13:0,C18:0,C20:2,C23:0,and C24:0(P<0.05),and negatively correlation with C17:1 and C18:1n9c(P<0.05).In summary,CIDEa and CIDEc genes play important roles in the regulation of intramuscular fat and fatty acids in Huanghuai sheep.
Keywords：Huanghuai sheepCIDETissue expressionIntramuscular fat
Publication Date：2025-02-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 131-137 )