Effects of LED Light Quality on Physiology,Biochemistry and Nutrient Content of Dendrobium officinale Test-tube Seedlings
LIN Qiongyu
ZHENG Lifang
MENG Xinya
WANG Jiangning
FU Qiunuan
SONG Xiqiang
Abstract：To investigate the impact of different LED light spectrums on the growth,photosynthesis,and nutrient accumulation of Dendrobium officinale test-tube seedlings,using D.officinale test-tube seedlings as the experimental material,six different light quality treatments were established[100%red light(R),100%blue light(B),80%red light+20%blue light(8R∶2B),70%red light+30%blue light(7R∶3B),and 50%red light+50%blue light(5R∶5B),white fluorescent light used as a control group(CK)].The growth indexes(plant height,leaf number,maximum leaf length,stem diameter,fresh weight,dry weight),antioxidant indexes[superoxide dismutase(SOD)activity,malondialdehyde(MDA)content,ascorbate peroxidase(APX)activity,peroxidase(POD)activity,catalase(CAT)activity)],photosynthetic indexes(chlorophyll a content,chlorophyll b content,chlorophyll a+b content,chlorophyll a/b ratio,carotenoids content),chlorophyll fluorescence indexes(Fv/Fm,ΦPSⅡ,ETRⅡ,qN,qP,Fv/Fo)and nutrient contents(amino acids,alkaloids,polysaccharides)were measured after 90 days of treatment.The results showed that the 8R∶2B treatment was beneficial to the elongation of plant height,leaf growth and the increase of stem diameter of D.officinale test-tube seedlings.The number of leaves and qN were highest in blue light treatment with 7.846,0.861.The antioxidant index was the best in 5R∶5B treatment as a whole,and the dry weight,fresh weight,nutrient content,chlorophyll content and chlorophyll fluorescence of test-tube seedlings were also the best in the 5R∶5B treatment.There were significant correlations among growth index,antioxidant enzyme index,nutrient index,chlorophyll fluorescence indexes and chlorophyll content.The results of principal component analysis showed that the physiological growth and nutrient accumulation of D.officinale test-tube seedlings with 5R∶5B treatment were the best,and the comprehensive score was 3.12.In conclusion,when the D.officinale test-tube seedlings are in the LED light quality environment of 5R∶5B treatment,it is most conducive to their growth and development and nutrient accumulation.
Keywords：Dendrobium officinaleLEDLight qualityPhysiology biochemistryNutrientChlorophyll fluorescence
Publication Date：2025-02-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：11( 66-76 )