Studies on Pollen Viability and Storage Characteristics of Yuluxiang Pear Hybrid Progeny

HUANG Ping; YANG Ce; PENG Jianying; ZHANG Yuxing

doi:10.15933/j.cnki.1004-3268.2025.01.014

Abstract：This study explored the variation characteristics of pollen fertility and pollen storage characteristics in pear hybrid progeny,to provide a basis for production application and theoretical research.The pollens from 20 strains of each hybrid progeny of Yuluxiang×Shinseiki and Yuluxiang× Xianghongli crosses were used as test materials.The fertility of the pollens was determined by determining the germination rate of different strains by in vitro culture,and the pollens of 3-4,7-9,6-17,10-4 and 6-3 strains were stored at-20 ℃ to observe the change of vigour during storage.The results showed that the pollen germination rates of 15 out of 20 strains of the Yuluxiang×Shinseiki cross progeny differed to different degrees of significance,and there were 5 sterile strains.The pollen viability of 7 out of 20 strains of Yuluxiang×Xianghongli cross progeny had different degrees of significant differences,and there were 13 sterile strains.Pollen viability after 13 months of storage was not significantly different from fresh pollen for 17 strains in the two cross combinations,while fresh pollen and pollen stored for 13 months were significantly different for 3 strains,with decreases of 34.91％for strain 10-173,55.48％for strain 3-4 and 100％for strain 2-13.The results of pollen viability dynamics of five strains of 3-4,7-9,6-17,10-4 and 6-3 stored at-20 ℃ showed that the pollen viability of 7-9,6-17 and 6-3 increased and then decreased with the prolongation of storage time,while the pollen viability of 3-4 showed no significant change in the first three months,and then began to decrease.Among the five strains,the pollen germination rate of 6-3,10-4,6-17,and 7-9 did not decrease compared with that of fresh pollen after 13 months of storage,while that of 3-4 was less than 50％of that of fresh pollen.There are significant differences in pollen viability to varying degrees and different proportions of sterile strains in the progeny of both the Yuluxiang×Shinseiki and Yuluxiang×Xianghongli crosses;the pollen viability of the progeny of the former combination is significantly higher than that of the latter combination,and the proportion of sterile strains is significantly lower than that of the latter combination.-20℃ low temperature condition is suitable for pear pollen storage for one year,and the pollen viability after storage can meet the pollination requirements.