Genetic Diversity Analysis and Fingerprint Construction of Henan Origanum vulgare and Its Related Species Origanum majorana by SRAP Markers
TIAN Huixin
YU Chan
YANG Fan
SU Ya'nan
WANG Xiaodong
ZHOU Yan
LI Hemin
HUANG Yong
LIANG Yan
XIA Zhi
Abstract：This study explored the genetic diversity and phylogenetic relationships of Origanum vulgare and its relative species Origanum majorana distributed in different regions of Henan Province,to provide a reference for the protection and development of O.vulgare and O.majorana germplasm resources.Using the sequence-related amplified polymorphism(SRAP)molecular marker method,7 wild populations of O.vulgare and 1 cultivated population,as well as 1 cultivated population of O.majorana,totaling 77 germplasm resources of 9 populations,were selected for genetic diversity analysis and construction of a DNA fingerprint map.The results indicated that 9 pairs of primers amplified a total of 98 bands,with 94 polymorphic bands,representing a polymorphism rate of 95.92％;on average,each pair of primers amplified 10.89 bands.The number of alleles(Na)was 1.959 2,the effective number of alleles(Ne)was 1.419 5,Nei gene diversity index(H)was 0.263 2,and Shannon's index(I)was 0.411 9.The UPGMA phylogenetic cluster diagram constructed based on SRAP molecular marker data showed that the genetic similarity coefficient ranged from 0.66 to 0.95.When the genetic similarity coefficient was 0.66,O.vulgare and O.major an a were divided into two separate branches.At a genetic similarity coefficient of 0.75,O.vulgare from different regions were divided into five branches.In summary,SRAP molecular markers can effectively distinguish between O.vulgare and O.majorana,and the genetic diversity of O.vulgare populations in Nanyang,Henan Province,is higher than that in other areas.Five pairs of SRAP primers(Me1/em6、Me1/em11、Me3/em4、Me3/em11、Me5/em3)are selected to construct DNA fingerprint maps for both O.vulgare and O.majorana germplasms.
Keywords：Origanum vulgareOriganum majoranaSRAPGenetic diversityDNA fingerprint
Publication Date：2025-01-14
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：11( 55-65 )