Analysis of Agronomic Traits and Physiological Mechanism of Maize Cytoplasmic Male Sterility Line C01

LIU Xinyi; LIAO Zhijie; LÜ Dan; MO Jiamei; JIA Wei; LUO Hongbing

doi:10.15933/j.cnki.1004-3268.2025.01.003

Abstract：In order to reveal the physiological mechanism of maize cytoplasmic male sterility,the agronomic traits and pollen morphology of cytoplasmic male sterility line C01 and maintainer line T01 were investigated,and the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)and the contents of malondialdehyde(MDA),soluble sugar,soluble protein and free proline in leaves were measured at seedling stage(V1),jointing stage(V6),11 leaves stage(V11),tasseling stage(VT),pollen dispersing stage(R1)and seed formation stage(R2),respectively.The results showed that there was no significant difference in agronomic traits between the sterile line C01 and the maintainer line T01,but there was significant difference in anther morphology.The anthers of T01 were full and normally dehiscent,light yellow,and there were pollens;the anthers of C01 were shriveled,non-dehiscent,tender green,the length and cross-sectional diameter of the anthers were significantly lower than those of T01,and there was no pollen.From V1 to VT,the soluble sugar content in C01 leaves was significantly lower than that in T01;Except for VT,the soluble protein content in C01 leaves was significantly lower than that in T01;The content of free proline in C01 leaves was significantly lower than that in T01 at VT and R1;At all growth stages,the SOD activity in C01 leaves was significantly lower than that in T01,there was no significant difference in POD activity,and the CAT activity was significantly lower than that in T01 at V11 and VT;There was no significant difference in MDA content.It is inferred that the synthesis and transport pathways of soluble sugar,soluble protein and free proline of C01 may be blocked,and the insufficient energy supply may lead to low CAT and SOD activity,which in turn leads to male sterility of C01.