Creation and Phenotypic Analysis of TaGW2-A1-Overexpression Transgenic Wheat Germplasm
ZHANG Haoran
LIU Xiaoying
ZHOU Fenglong
XU Mingchen
GUO Zhengzheng
CHENG Jiayu
ZHANG Kunpu
WANG Daowen
SHI Cuilan
Abstract：The expression vector of TaGW2-A1 was constructed,and transformed into wheat;T2 homozygous transgenic lines were identified and screened;the expression of TaGW2-A1 gene of the homozygous transgenic line was analyzed by real-time fluorescent quantitative PCR and Western blot;subcellular localization analysis was performed by tobacco transient expression and cell component separation;the agronomic traits and quality traits of homozygous lines were analyzed,so as to lay foundation for further analysis of TaGW2 regulatory network,and provide gene resource for breeding new wheat varieties with high yield and quality.The results showed that the expression vector of TaGW2-A1 gene was successfully constructed,positive transgenic plants were obtained,and homozygous transgenic lines were screened in T2 generation.The transcription level of TaGW2-A1 was significantly higher in leaves and grains of homozygous transgenic lines,but TaGW2-A1 protein was detected only in grains.Subcellular localization results showed that TaGW2-A1 protein mainly functioned in cytoplasm and nucleus.Phenotypic analysis results showed that TaGW2-A1 overexpression significantly reduced plant height,grain length,grain width,1 000-grain weight and yield per plant,but had no significant effect on grain number per spike and grain protein content.
Keywords：TaGW2-A1WheatThousand-grain weightGrain widthOverexpression
Publication Date：2025-01-14
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：8( 1-8 )