Analysis of Antibacterial Activity and Secondary Metabolites of Paenibacillus kribbensis against Gram-negative Bacteria
LI Haili
XU Yindi
WANG Zhifang
ZHU Wenhao
CHEN Jianhao
LI Bin
DUAN Jin'gang
FENG Lili
YANG Fan
MA Chunjiang
YAN Xiangzhou
Abstract：In order to screen the endophytes of Paeonia lactiflora Pall with good antibacterial activity against gram-negative bacteria,the endophytes of P.lactiflora which have antagonistic effect on Escherichia coli and Salmonella were isolated by surface disinfection and plate face-off method,and its antibacterial effect and secondary metabolites were studied.The species was identified by morphological observation and 16S rRNA gene sequencing.20 strains of endophytic bacteria were isolated from different tissues such as root,stem and leaf.A gram-negative endophyte HNYJ291 with good antibacterial effect was obtained from 20 endophyte strains by plate confrontation assay,which was identified as Paenibacillus kribbensis by morphological observation and molecular analysis.The bacteria could effectively inhibit E.coli and Salmonella.Whole genome sequencing analysis showed that the genome size of strain HNYJ291 was 5 609 136 bp,the GC content was 45.48%,and the number of coding genes was predicted to be 4 805.4 779,3 678,4 027,3 941,2 994 and 2 700 genes were annotated by NR,Swiss-Prot,Pfam,COG,GO and KEGG databases,respectively,and 10 gene clusters related to secondary metabolite synthesis were predicted by antiSMASH.The main secondary metabolites included cyclolipopeptides antibiotics(fusaricidin B),bactericin(paenilan),antimicrobial lipids(tridecaptin),polymyxin and antimicrobial peptides(paenicidin A),etc.From gene clusters related to antibacterial active substances in its genome,it can be predicted that this strain has the potential to develop new drugs and be used as a feedable microorganism.
Keywords：Paenibacillus kribbensisPaeonia lactiflora PallEndophyteSalmonellaEscherichia coliBacteriostatic effectAntibacterial active substance
Publication Date：2024-10-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：11( 159-169 )