Preparation of Monoclonal Antibody against Major Capsid Protein of Largemouth Bass Iridovirus and Its Epitope Identification
SONG Zhanlin
MING Shengli
ZENG Lei
PAN Jiajia
ZHAO Liming
LIU Taoxue
WANG Jiang
LIU Zhonghu
Abstract：The major capsid protein(MCP)was selected as the target protein of largemouth bass iridovirus(LMBV),MCP recombinant protein was expressed by prokaryotic expression system and used to immunize BALB/c mice.The immunized mice spleen B lymphocytes were fused with mouse myeloma cells(SP/20),and monoclonal antibodies were obtained through subclonal screening,abdominal inoculation and collection of ascites.The specificity of the monoclonal antibody and its epitopes were studied by Western blot and immunohistochemistry.The results showed that one monoclonal antibody strain 2C9-6 was obtained.The specificity analysis results suggested that 2C9-6 could specifically recognize eukaryotically expressed LMBV-MCP and LMBV virus particles.Epitope analysis results showed that the epitope recognized by 2C9-6 was located in the peptide segment composed of amino acids at positions 1-20 of the N terminal of LMBV-MCP protein.
Keywords：Largemouth bass iridovirus(LMBV)Main capsid protein(MCP)Monoclonal antibodyEpitope
Publication Date：2024-10-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：10( 149-158 )