Composition Analysis of Leaves and Latex of Euphorbia maculata L.Based on Non-Targeted Metabolomics
MA Liang
ZHOU Yan
GAO Qi
HUANG Yong
XIA Zhi
LI Hemin
Abstract：To compare the difference of active ingredients in the leaves and latex of Euphorbia maculata L.,relevant metabolite data were collected by liquid chromatograph mass spectrometer(LC-MS),and the chemical ingredients of the leaves and latex of Euphorbia maculata L.were analyzed based on non-targeted metabolomics.Principal component analysis,multivariate statistical analysis,and orthogonal partial least squares discriminant analysis were used to identify differential metabolites.The pharmacological activities of major metabolites in leaves and latex were compared by literature search.The results showed that a total of 1 229 metabolites were identified in the leaves and latex of Euphorbia maculata L.,mainly including alkaloids(217),phenylpropanoids and their derivatives(178),lipids(147),terpenes(137),flavonoids(113)and other compounds.859 differential metabolites were screened through multivariate statistical analysis,of which 466 metabolites were highly expressed in latex.Among them,14 metabolites,including 3-dehydroshikimic acid(phenolic acid),sorbic acid(terpene),protocatechuic acid(phenolic acid),cinnamaldehyde(phenylpropanoid and its derivatives),hesperidin(flavonoid),ligustrolacton(terpene),α-asarone(phenylpropanoid and its derivatives),salicylic acid(phenolic acid),D-camphor(terpene),Linarin(flavonoid),trans-ferulic acid(phenylpropanoid and its derivatives),curcumin(phenolic acid),ethyl eicosapentaenoic acid(lipid),β-caryophyllene(terpene),etc.,were reported in Euphorbia maculata L.for the first time detected in latex.The expression levels of flavonoids in the leaves were significantly higher than those in the latex;The expression levels of ethyl gallate,ethyl protocatechuate and trans-ferulic acid in latex were significantly higher than those in leaves,with trans-ferulic acid expressing 57.74 times more in latex than in leaves.In conclusion,there is a significant difference in the metabolite content between the leaves and the latex of Euphorbia maculata L.
Keywords：Euphorbia maculata L.Euphorbiae humifusae HerbaLeafLatexMetabolomeActive ingredient
Publication Date：2024-10-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：14( 82-95 )