Establishment of Callus Induction and Regeneration System of Cyperus esculentus
ZHU Yajing
ZHANG Xiangge
XU Xinran
HU Haiyan
CHENG Shan
CHEN Chen
LI Chunxin
SONG Wanxian
SONG Wenxu
WANG Huiwei
Abstract：In order to establish an efficient callus induction and regeneration system of Cyperus esculentus,the explants(young root,young leaf,young leaf sheath and tillering node)for callus induction were screened,and the effects of different plant growth regulator combinations on callus induction,proliferation and differentiation were explored.The results showed that the best explant for callus induction of Cyperus esculentus was tillering node;the optimal plant growth regulator combination for callus induction was 2.5 mg/L 2,4-D+0.6 mg/L 6-BA+0.6 mg/L NAA+0.9 mg/L ZT,and the induction rate could reach 65.58%;the optimal plant growth regulator combination for callus proliferation was 0.5 mg/L 2,4-D+0.9 mg/L 6-BA+1.2 mg/L NAA+1.2 mg/L ZT,and the proliferation rate could reach 68.12%;the optimal plant growth regulator combination for callus differentiation was 0.9 mg/L 6-BA+0.6 mg/L NAA,and the differentiation rate could reach 66.67%.In this study,the best explants suitable for callus induction of Cyperus esculentus were screened,and the conditions of callus induction,proliferation and differentiation of Cyperus esculentus were optimized,which could lay a foundation for the subsequent research and breeding application of biotechnology such as somatic hybridization and genetic transformation of Cyperus esculentus.
Keywords：Cyperus esculentusCallusExplantPlant growth regulatorRegeneration system
Publication Date：2024-10-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：9( 73-81 )