Comparative Analysis of Chromosome Sets Characteristics of Sesame Cultivated Species and Wild Species Based on FISH and GISH Technologies
MA Qin
ZHAO Ruihong
JU Ming
CHEN Chengbin
DUAN Yinghui
YANG Weifei
MIAO Hongmei
ZHANG Haiyang
Abstract：To reveal the evolutionary characteristics of Sesamum,explore the genome structure revolution and species evolution,and promote the development and utilization of wild resources,the cultivated species S.indicum(var.Yuzhi 11)and the 2n=26 type wild species S.alatum(var.3651)were used as experimental materials,fluorescence in situ hybridization(FISH)and genome fluorescence in situ hybridization(GISH)techniques were used to analyze the chromosome sets characteristics of sesame cultivated species and wild species.The results showed that both the cultivated species Yuzhi 11 and the wild species S.alatum(var.3651)were 2n=2x=26 karyotype;the rDNA-FISH hybridization results indicated that in the 13 pairs of chromosomes of the cultivated species Yuzhi 11,3 pairs of chromosomes(the 7th,8th and 9th pairs)had 45S rDNA-specific signals at the short arm tips,which were displayed as satellite specific chromosome.At the same time,2 pairs of chromosomes(the 5th and 11th pairs)had 5S rDNA-specific signals on the short arms,with the 5S rDNA and 45S rDNA signals located on different chromosomes.In the wild species S.alatum(var.3651),2 pairs of chromosomes(the 4th and 7th pairs)carried 45S rDNA hybridization signals and 1 pair of chromosome(the 4th pair)carried 5S rDNA specific signals,with the 5S rDNA and 45S rDNA signals located on the same chromosome but at different positions.This indicated that there were significant differences in chromosome characteristics between the cultivated species and the wild species S.alatum(var.3651).The GISH hybridization results showed that when using the genomic DNA of the cultivated species Yuzhi 11 and the wild species S.alatum(var.3651)as probes for self-hybridization,each chromosome carried hybridization signals of varying strengths,while hybridization with the other's chromosomes showed very few hybridization signals.The cultivated species and the wild species S.alatum(var.3651)have the same number of chromosomes,but there are clear differences in the quantity,distribution of rDNA,and GISH signal sites,indicating that the 2n=26 type cultivated species and wild species have distant phylogenetic relationship.
Keywords：SesameCultivated speciesWild species S.alatumChromosome setrDNAFISHGISH
Publication Date：2024-10-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 48-53 )