Establishment of Indirect ELISA Antibody Detection Method Based on Penton Protein of Fowl Adenovirus Serotype 4
LI Peng
LEI Mengyao
FENG Lili
WANG Zhenwei
GUAN Chunxiao
ZHENG Hongshuang
WANG Junru
WANG Liping
LI Yanjin
WU Xinyuan
LIU Xingyou
JIN Qianyue
Abstract：Penton protein is the main structural protein that constitutes the capsid of fowl adenovirus serotype 4(FAdV-4),which has high conservation and good immunogenicity.An indirect ELISA antibody detection method based on the purified Penton protein of FAdV-4 was established.The results showed that the optimal coating concentration of Penton protein was 2 µg/mL,the serum dilution multiple was 200 times,the enzyme-labeled antibody dilution multiple was 1∶5 000,the positive critical value was 0.222,and the sensitivity could reach 1∶6 400.There was no cross-reaction with infectious bronchitis virus,infectious bursal disease virus,and infectious laryngotracheitis virus,and the specificity was good.The coefficient of variation of inter-batch and intra-batch repeatability was less than 10%.The positive detection rate in 100 clinical serum samples was 77%,and the coincidence rate with the commercial kit was 98%.In summary,the ELISA antibody detection method established in this study can be used for the clinical detection of antibodies against FAdV-4.
Keywords：Fowl adenovirus serotype 4Penton proteinIndirect ELISAAntibody detection
Publication Date：2024-07-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：9( 133-141 )