Effects of BmNPV Infection on β-N-acetylglucosidase Activity and Transcriptional Level of Related Genes in Bombyx mori
YANG Weike
ZHANG Lusi
FAN Yonghui
LIU Zenghu
TANG Fenfen
Abstract：In order to further analyze the action mechanism of β-N-acetylgucosaminidase(GlcNAcases)during Bombyx mori nuclear polyhedrovirus(BmNPV)infection,the expression of GlcNAcases genes was detected by fluorescence quantitative PCR and the activity of GlcNAcases was determined using the enzyme activity assay kit in heamolymph,fat body and midgut of silkworm larvae after oral infection with BmNPV at 3,6,9,12 and 24 h.The results indicated that the GlcNAcases activity in hemolymph,fat body and midgut showed a trend of gradual increase and then sharp decrease,while the transcription levels of the four GlcNAcases coding genes were different in these tissues.In hemolymph,BmGlcNAcase1 and BmGlcNAcase2 were significantly up-regulated at 3 h to 12 h after infection with BmNPV,while the expression levels of BmGlcNAcase3 and BmGlcNAcase4 showed no significant change compared with the control group(no infection).At 24 h,the expression of four GlcNAcases genes began to down-regulate and the expression levels were significantly lower than those in the control group.After infection with BmNPV,the expression of BmGlcNAcase2 and BmGlcNAcase3 in fat body was first up-regulated and then down-regulated.BmGlcNAcase2 began to up-regulate at 3 h and BmGlcNAcase3 began at 9 h.In addition,the expression levels of BmGlcNAcase1 and BmGlcNAcase4 did not change significantly.In midgut,BmGlcNAcase2 and BmGlcNAcase4 were gradually up-regulated firstly,their expression levels were gradually increased and showed a peak,then sharply decreased and the expression was significantly inhibited.However,BmGlcNAcase1 and BmGlcNAcase3 were down-regulated continuously and their expression levels gradually decreased after infection with BmNPV.In conclusion,β-N-acetylglucosidases encoded by BmGlcNAcase2 may play a certain immune defense function in the process of silkworm resisting BmNPV infection.
Keywords：Bombyx mori nuclear polyhedrovirusβ-N-acetylglucosidasesBombyx moriEnzymatic activityGene expression
Publication Date：2024-05-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 150-156 )