Optimization of Oudemansiella raphanipes Liquid Fermentation Medium
LIU Guoli
CHEN Xun
XIAO Jun
MA Xiaoying
ZHANG Min
ZHANG Peng
ZHANG Man
GONG Na
Abstract：Oudemansiella raphanipes strain H1 was used as experimental material and single factor test,Plackett-Burman design,maximum climb test and Box-Benhnken design response surface method were used to optimize the liquid culture medium of strain H1 with mycelial dry weight as the main evaluation index,in order to improve the mycelium biomass of O.raphanipes liquid seeds,and provide the guarantee for the industrial large-scale production of O.raphanipes.The results showed that the most suitable carbon source for the growth of O.raphanipes was corn meal and the most suitable nitrogen source was soybean meal.When the rotating speed of shaker was 150 r/min and the culture temperature was 25 ℃,the formula of optimized liquid fermentation medium was 25.8 g/L glucose,26.0 g/L corn flour,1.0 g/L KH2PO4,1.5 g/L soybean meal,1.0 g/L yeast extract powder,0.4 g/L MgSO4,0.01 mg/L VB1,and under this culture condition,the measured value of mycelium dry weight was 23.2 g/L.The culture cycle was shortened by 3 d,and the mycelial activity was higher compared with conventional medium.
Keywords：Oudemansiella raphanipesPlackett-Burman experimentResponse surface methodLiquid spawnFermentation medium
Publication Date：2024-05-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：8( 133-140 )