Expression and Bioinformatics Analysis of Aeromonas hydrophila Outer Membrane TolB Protein
CHEN Tianmeng
CAI Tongxuan
WANG Jialu
TIAN Muye
ZHAO Baohua
LIU Dong
Abstract：The purpose of this study is to evaluate the application of TolB as the candidate vaccine antigen to prevent Aeromonas hydrophila infection in fish.Outer membrane protein gene tolB from A.hydrophila was cloned and expressed in Escherichia coli BL21(DE3).Physicochemical properties and advanced structures of TolB protein were analyzed by bioinformatics.The results indicated that tolB gene had an open reading frame of 1263 bp(exclude signal peptide),encoding a protein(TolB)of 420 amino acids with molecular mass of 45.78 ku.TolB was successfully expressed and purified from Escherichia coli BL21(DE3).TolB was a stable and hydrophilic outer membrane protein.TolB had homology among different bacteria,and was more closely related in Aeromonas.The secondary structure of TolB was dominated by random coil.TolB had potential B,CTL and Th cell antigen epitopes and interacted with other Tol-Pal system proteins.In conclusion,TolB could be used as an effective vaccine candidate antigen agaisnt A.hydrophila.
Keywords：Aeromonas hydrophilaTolBProkaryotic expressionProtein purificationBioinformatics
Publication Date：2024-02-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：8( 136-143 )