Prokaryotic Expression of Vsp Protein and Preparation of Monoclonal Antibody against Mycoplasma bovis
XU Jia
SUN Huaichang
Abstract：In order to develop a rapid diagnostic reagent for Mycoplasma bovis,the monoclonal antibody against CaIgV-Vsp protein was prepared after expressing recombinant CaIgV-Vsp protein in the prokaryotic expression system of Escherichia coli.The results showed that the recombinant plasmid pET-CaIgV-Vsp was successfully constructed.SDS-PAGE analysis showed that the fusion protein was soluble protein.The results of ELISA and Western blot showed that the recombinant CaIgV-Vsp protein could react specifically with the positive serum of Mycoplasma bovis and had good immunogenicity.Four hybrioma cell lines secreting monoclonal antibodies against CaIgV-Vsp protein were prepared successfully.The ELISA titers of ascites were 5.2×105,1.3×105,2.6×105 and 2.6×105,respectively.
Keywords：Mycoplasma bovisVariable surface lipoprotein(Vsp)Prokaryotic expressionMonoclonal antibody
Publication Date：2023-12-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 142-148 )